So, for my 'suppose to be' last semester exam date is like that... this early November. It was out long time already and I think a lot of ... words came out from my mouth due to that 9,10,11 paper.... Its tough... and yeah it is... I usually will take a break after done with a paper.... then how about this one??? So, the stratergy needed for this semester is the CBC technique for sequencing DNA.... biotech last sem student jer fhm kot.... theoretically there are two common technique to do DNA sequencing... for some Layman outside... have you heard about HUGO? or HUman Genome Sequencing? Sanger Method??? ok, basically the techniques was a procedure to retrieve DNA sequence. those 2 techniques are WGS and CBC.
WGS or "Whole Genome Sequence" is a technique that what my discussion group labeled it as -senang2 dulu susah2 kemudian- since it just sheared the DNA and put it all away in the plasmid to be transformed into host (E.coli) then clone it then sequence it.... why it senang2 dulu? becoz it juz sheared, ligate it in plasmid and simply clone the fragment without considering the presence of repetitive DNA sequence in living organisms....
Do you know??? only 4% of total human genomes which was about 20000bp (correct me if Im wrong) is what we so called functional genes (genes that encode protein) and another 96% is those introns, repetitive DNA and junk DNA??? simple word, human juz need 500bp of DNA if we want to omtimize DNA function in human?? but that is not simply just like that.... why? because thats is current knowledge of human now.... those repetitive may have functions in human (currently only being used for DNA fingerprinting+ telomeres act in DNA aging+etc)... n those what we so call 'junk DNA' when it was dipose, it effect human living??? (x tau... x bace journal gie kot ttg nie:P)
What a craps Im talking about??? ok, another technique was CBC or clone by clone..... where this technique after shearing the chromosome into short fragment it was ligate into BAC (Bacteria Artificial Chromosome)... the difference is BAC can accomodate larger DNA framgent size and it has some markers to indicates it positioning to make work wasy in physical mapping of BAC through chromosome walking... after done with clonong BACs... a procedure of WGS was done for each BAC after the physical mapping assemble done.... n arrrrggh, nak tau ntuk bdk biotech monash nanti2 la korg blaja... yang batch ak, u suppose to know this more than me or maybe same with me la kan;).... yang rase cam x fhm tuh.... hopefully u can get some view what this biotechnologist student studying for...
K, sambung studi(~~)
WGS or "Whole Genome Sequence" is a technique that what my discussion group labeled it as -senang2 dulu susah2 kemudian- since it just sheared the DNA and put it all away in the plasmid to be transformed into host (E.coli) then clone it then sequence it.... why it senang2 dulu? becoz it juz sheared, ligate it in plasmid and simply clone the fragment without considering the presence of repetitive DNA sequence in living organisms....
Do you know??? only 4% of total human genomes which was about 20000bp (correct me if Im wrong) is what we so called functional genes (genes that encode protein) and another 96% is those introns, repetitive DNA and junk DNA??? simple word, human juz need 500bp of DNA if we want to omtimize DNA function in human?? but that is not simply just like that.... why? because thats is current knowledge of human now.... those repetitive may have functions in human (currently only being used for DNA fingerprinting+ telomeres act in DNA aging+etc)... n those what we so call 'junk DNA' when it was dipose, it effect human living??? (x tau... x bace journal gie kot ttg nie:P)
What a craps Im talking about??? ok, another technique was CBC or clone by clone..... where this technique after shearing the chromosome into short fragment it was ligate into BAC (Bacteria Artificial Chromosome)... the difference is BAC can accomodate larger DNA framgent size and it has some markers to indicates it positioning to make work wasy in physical mapping of BAC through chromosome walking... after done with clonong BACs... a procedure of WGS was done for each BAC after the physical mapping assemble done.... n arrrrggh, nak tau ntuk bdk biotech monash nanti2 la korg blaja... yang batch ak, u suppose to know this more than me or maybe same with me la kan;).... yang rase cam x fhm tuh.... hopefully u can get some view what this biotechnologist student studying for...
K, sambung studi(~~)
-RedBloodCell-
2 comments:
mmg kne triple kill lah ko...haha..nasib baik bkn ultra kill.
all da best! triple kill skali ngan triple energy so nak makan triple pun x pe! jangan triple tdo!
skian trima kaseh
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